The following supplements have been selected for their safety and efficacy profile in the pediatric population, as well as for their specific action in modulating the microbiota and intestinal health.

Treating dysbiosis in children requires a carefully balanced approach that combines the gentle elimination of pathogens with the restoration of beneficial gut flora and intestinal repair. This protocol is designed so that each component works in harmony, maximizing the child's well-being.

• "Clean and Prepare the Ground" (Dysbiosis Control Formula + NAC): Imagine your child's gut as a garden with some "weeds" (pathogenic microorganisms) growing too large. The **Dysbiosis Control Formula** (Oregano Oil, Tributyrin, DGL, Xylitol) acts as a "gentle gardener" that helps control the growth of these weeds. Oregano Oil directly reduces the pathogens, Xylitol inhibits them, and DGL, along with Tributyrin, begins to nourish and repair the soil (the intestinal lining). **NAC** is the "structure breaker" that disarms the defenses (biofilms) that the weeds use to hide, allowing the gardener to work more effectively. This phase prepares the environment for the "good plants" to grow.
• "Sowing Good Seeds and Strengthening the Soil" (Saccharomyces Boulardii + Reuteri + Lactobacillus Rhamnosus + PHGG): Once the ground is clearer, it's crucial to sow abundantly with "good seeds" (probiotics and prebiotics). **Saccharomyces Boulardii**, **Reuteri**, and **Lactobacillus Rhamnosus** are our star probiotic strains. They are like the "native plants" that repopulate the garden, competing with weeds for space and nutrients, and producing their own "protective substances." Each has a unique role: *S. Boulardii* is robust against toxins and supports overall intestinal function; *Reuteri* has a targeted antimicrobial action and improves motility; *L. Rhamnosus* is excellent for strengthening the intestinal barrier and modulating immunity. **PHGG** is the mild, well-tolerated "fertilizer" that selectively feeds these beneficial plants, helping them to grow strong and produce more healthy substances like butyrate.
• "Nourishing Defenses and Protecting the Garden" (Lactoferrin + Colostrum): Finally, we need to strengthen the garden's intrinsic defenses and ensure its long-term well-being. Lactoferrin and colostrum are the "immune guardians" and "repairers" of our gut. Lactoferrin helps regulate iron, a nutrient that pathogens need, and supports immunity. Colostrum is a rich source of antibodies and growth factors that act as direct "shields" against threats and as "builders" that repair any damage to the intestinal wall. This duo improves the resilience of the child's gut, helping it to better defend itself against future attacks and maintain a healthy balance.

In summary, this comprehensive protocol for dysbiosis in children not only aims to reduce imbalanced microbial populations, but also simultaneously focuses on restoring beneficial flora, healing and strengthening the intestinal barrier, and modulating the immune response. It is a gentle and multifaceted approach that supports the child's digestive and overall health, promoting a healthy gut environment for their growth and development.

To maximize effectiveness and avoid potential interactions, it is important to consider the timing of administration of each supplement.

• Probiotics (Saccharomyces Boulardii, Reuteri, Lactobacillus Rhamnosus): It is ideal to take them on an empty stomach or at least 30 minutes before meals . Avoid taking them with the Dysbiosis Control Formula, as the antimicrobials could affect the viability of the probiotic strains. They can be taken together.
• Dysbiosis Control formula in drops: It is best taken with meals to reduce any potential gastrointestinal irritation. It can be taken in conjunction with NAC and Lactoferrin + Colostrum.
• PHGG: It can be mixed with juice or water and taken at any time of day, ideally **away from other supplements that require maximum absorption**, as, being fiber, it could slightly reduce absorption. A good time is before bed or mid-morning/afternoon.
• NAC: Can be taken with the Dysbiosis Control Formula. If used for biofilm disruption, it may be beneficial to take it **on a relatively empty stomach** (e.g., between meals).
• Lactoferrin + Colostrum: These can be taken with meals or on an empty stomach. They are compatible with most supplements, but if the child is sensitive, giving them with a meal may be better.

These supplements can be considered to enhance the results of the protocol, especially in cases of more persistent dysbiosis or when additional support for digestive function and intestinal recovery is sought.

• Zinc Carnosine: For intensive repair of the intestinal mucosa. Its reparative and anti-inflammatory properties are excellent for a compromised gut.
• N-Acetylglucosamine (NAG): A precursor of intestinal mucus, useful for repairing the mucosal lining and strengthening the intestinal barrier.
• Magnesium (well-tolerated forms such as citrate or bisglycinate): Useful for constipation associated with dysbiosis, as it helps relax intestinal muscles and draw water into the colon. Also crucial for overall nerve and muscle function.
• Vitamin D3 + K2: Essential for immune modulation and bone health, both crucial in children with chronic dysbiosis who may have compromised absorption.
• Omega-3 (EPA/DHA): Powerful anti-inflammatories, important for reducing systemic and brain inflammation that often accompanies dysbiosis and can affect behavior and cognition.

The long-term success of the protocol is optimized with lifestyle changes that support the child's gut and overall health. These recommendations are essential for creating an environment conducive to microbial balance and well-being.

Nutrition: Mindful Nutrition

• A diet rich in whole foods: Prioritize fruits, vegetables, good-quality lean protein, healthy fats, and whole grains (if well tolerated). Limit processed foods, added sugars, and refined carbohydrates, which can feed pathogens.
• Reduce Inflammatory Foods: Identify and reduce common food allergens or sensitivities (e.g., dairy, gluten, soy, corn) that may contribute to intestinal inflammation and dysbiosis. A supervised elimination-reintroduction diet may be helpful.
• Incorporate Fermented Foods (if tolerated): Small amounts of naturally fermented foods such as water kefir, sauerkraut, kimchi, or unsweetened yogurt can introduce beneficial bacteria. Start with very small portions.
• Promote Fiber Diversity: Offer a wide variety of fruits, vegetables and legumes (if tolerated) to nourish a diverse microbiota.

Rest and Quality Sleep

• Establish a Regular Sleep Routine: Ensure your child gets the recommended amount of sleep for their age (generally 9-12 hours for school-aged children). Adequate sleep is vital for cell repair, immune modulation, and digestive function.
• Optimal Sleep Environment: Create a dark, quiet and cool bedroom, free of screens before going to sleep.

Stress Management and Emotional Regulation

• Play and Relaxation: Stress directly affects the gut-brain axis. Encourage free play, time in nature, creative activities, and simple relaxation techniques (e.g., deep breathing, children's yoga) to help children manage stress.
• Connection and Security: A safe and emotionally supportive family environment is crucial for a child's health.

Regular Physical Activity

• Encourage Daily Movement: Moderate and regular physical activity improves bowel motility, reduces stress, and supports overall health. Avoid sedentary behavior and promote active outdoor play.

Other Useful Practices

• Adequate Hydration: Ensure the child drinks enough pure water throughout the day.
• Oral Hygiene: Maintain good oral hygiene, as oral dysbiosis can influence intestinal dysbiosis.
• Time in Nature and Contact with Pets: Exposing children to a natural environment and contact with animals (if appropriate) can enrich the diversity of their microbiota.

Implementing this protocol in children requires careful supervision and consideration of the specific circumstances of each case. The following warnings are important:

• Age and Weight: Doses must be strictly adjusted to the child's weight and age. The dosages given here are general guidelines and must be adjusted individually.
• Sensitivity/Allergy Reactions: Be alert for any signs of allergic reaction or intolerance to the supplements (e.g., skin rashes, worsening of digestive symptoms).
• Dysbiosis Control Formula (Oregano Oil): Although diluted, oregano oil can be potent. Monitor for any gastrointestinal irritation. Do not administer to children with active ulcers or severe reflux without extreme caution.
• PHGG: If introduced too quickly, it may cause bloating or gas in some sensitive infants. Increase the dose very gradually.
• NAC: In rare cases, it may cause nausea or stomach upset. Ensure it is thoroughly dissolved if the capsule is opened.
• Lactoferrin + Colostrum: Although generally well tolerated, if the child has a severe cow's milk protein allergy, bovine colostrum could be problematic. Look for lactose-free colostrum or alternatives.
• Drug Interactions: Although natural supplements generally have fewer interactions than drugs, always consider whether the child is taking other medications and consult sources of information on possible interactions.
• Herxheimer reaction (Die-Off): At the start of the protocol or when increasing the dose, the child may experience a temporary worsening of symptoms (fatigue, irritability, increased gas, or changes in bowel movements). This indicates the release of toxins by the dying microorganisms. It is important to explain this to the parents so they are not alarmed and to ensure adequate hydration.
• Continuous Monitoring: It is essential to closely monitor the child's symptoms, stool consistency, sleep patterns, behavior, and overall well-being to adjust the protocol as needed.